期刊
VIROLOGY
卷 301, 期 2, 页码 212-225出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/viro.2002.1595
关键词
cytochalasin D; latrunculin A; atomic force microscopy
类别
Morphogenesis of influenza virus is a poorly understood process that produces two types of enveloped virion: similar to 100-nm spheres and similar diameter filaments that reach 20 mum in length. Spherical particles assemble at plasma membrane lipid rafts in a process independent of microfilaments, The budding site of filamentous virions is hitherto uncharacterised but their formation involves the actin cytoskeleton. We confirm microfilament involvement in filamentous budding and show that after disruption of cortical actin by jasplakinolide, HA, NP, and M1 redistributed around beta-actin clusters to form novel annular membrane structures. HA in filamentous virions and jasplakinolide-induced annuli was detergent insoluble at 4degreesC. Furthermore, in both cases HA partitioned into low buoyant density detergent-insoluble glycolipid domains, indicating that filamentous virions and annuli contain reorganised lipid rafts, We propose that the actin cytoskeleton is required to maintain the correct organisation of lipid rafts for incorporation into budding viral filaments. (C) 2002 Elsevier Science (USA).
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