期刊
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
卷 283, 期 4, 页码 C1313-C1323出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00182.2002
关键词
ion channels; volume regulation; cancer cells
Patchclamping and cell image analysis techniques were used to study the expression of the volume-activated Cl- current, I-Cl(vol), and regulatory volume decrease (RVD) capacity in the cell cycle in nasopharyngeal carcinoma cells (CNE-2Z). Hypotonic challenge caused CNE-2Z cells to swell and activated a Cl- current with a linear conductance, negligible time-dependent inactivation, and a reversal potential close to the Cl- equilibrium potential. The sequence of anion permeability was I- > Br- > Cl- > gluconate. The Cl- channel blockers tamoxifen, 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), and ATP inhibited I-Cl(vol). Synchronous cultures of cells were obtained by the mitotic shake-off technique and by a double chemical-block (thymidine and hydroxyurea) technique. The expression of I-Cl(vol) was cell cycle dependent, being high in G(1) phase, downregulated in S phase, but increasing again in M phase. Hypotonic solution activated RVD, which was cell cycle dependent and inhibited by the Cl- channel blockers NPPB, tamoxifen, and ATP. The expression of I-Cl(vol) was closely correlated with the RVD capacity in the cell cycle, suggesting a functional relationship. Inhibition of I-Cl(vol) by NPPB (100 muM) arrested cells in G(0)/G(1). The data also suggest that expression of I-Cl(vol) and RVD capacity are actively modulated during the cell cycle. The volume-activated Cl- current associated with RVD may therefore play an important role during the cell cycle progress.
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