Further isolation of peroxynitrite and 1,1-diphenyl-2-picrylhydrazyl radical scavenging isorhamnetin 7-O-glucoside from the leaves of brassica juncea L.

From the leaves of Brassica juncea, an radical scavenging isorhamnetin 7-O-glucoside on peroxynitrite and 1,1-diphenyl-2-picrylhydrazyl (DPPH) was isolated and characterized based on the spectroscopic evidence. The compound showed the peroxynitrite and DPPH scavenging activities with IC50 values of 2.07 +/- 0.17 and 13.3 muM, respectively. Penicillamine and L-ascorbic acid as positive control exhibited radical scavenging activities with IC50 values of 3.17 +/- 0.39 and 12.78 muM, respectively.