期刊
BIOLOGICAL CHEMISTRY
卷 383, 期 10, 页码 1553-1564出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/BC.2002.175
关键词
catalytic selectivity; cytochrome P450; kinetics; rate-limiting steps
资金
- NCI NIH HHS [R01 CA90426, R35 CA44353] Funding Source: Medline
- NIEHS NIH HHS [P30 ES00267] Funding Source: Medline
Cytochrome P450 (P450) reactions are of interest because of their relevance to the oxidative metabolism of drugs, steroids, carcinogens, and other chemicals. One of the considerations about functional characterization is which steps of the catalytic cycle are rate-limiting. Detailed analysis indicates that several different steps can be rate-limiting with individual P450 reactions. N-Dealkylation of para-substituted N,N-dimethylanilines is a function of the electron withdrawing/donating properties of the substituent and the oxidation-reduction potential of the substrate, supporting a role in rate-limiting electron transfer from substrate to the high valent P450. In the oxidations of ethanol and acetaldehyde by human P450 2E1, a step following product formation must be the slow step (but not product release per se). Several oxidations catalyzed by human P450s 1A2 and 2D6 show slow C-H bond breaking, and apparent high-valent iron complexes accumulate in the reaction steady-state. Kinetic simulations were used to test the suitability of potential schemes and to probe the effects of changes in individual reaction steps.
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