Effects of heat stress on photosynthetic electron transport in a marine cyanobacterium Arthrospira sp.

Arthrospira (Spirulina) is widely used as human health food and animal feed. In cultures grown outdoors in open ponds, Arthrospira cells are subjected to various environmental stresses, such as high temperature. A better understanding of the effects of high temperature on photosynthesis may help optimize the productivity of Arthrospira cultures. In this study, the effects of heat stress on photosynthetic rate, chlorophyll a fluorescence transients, and photosystem (PS) II, PSI activities in a marine cyanobacterium Arthrospira sp. were examined. Arthrospira cells grown at 25 A degrees C were treated for 30 min at 25 (control), 30, 34, 37, or 40 A degrees C in the dark. Heat stress (30-37 A degrees C) enhanced net photosynthetic O-2 evolution rate. Heat stress caused over-reduction PSII acceptor side, damage of donor side of PSII, decrease in the energetic connectivity of PSII units, and decrease in the performance of PSII. When the temperature changed from 25 to 37 A degrees C, PSII activity decreased, while PSI activity increased, the enhancement of photosynthetic O-2 evolution was synchronized with the increase in PSI activity. When temperature was further increased to 40 A degrees C, it induced a decrease in photosynthetic O-2 evolution rate and a more severe decrease in PSII activity, but an increase in PSI activity. These results suggest that PSI activity was the decisive factor determining the change of photosynthetic O-2 evolution when Arthrospira was exposed to a temperature from 25 to 37 A degrees C, but then, PSII activity became the decisive factor adjusting the change of photosynthetic O-2 evolution when the temperature was increased to 40 A degrees C.