期刊
STRUCTURE
卷 10, 期 10, 页码 1317-1328出版社
CELL PRESS
DOI: 10.1016/S0969-2126(02)00827-4
关键词
tubulin; microtubules; electron crystallography; protein structure; dynamic instability
资金
- NIGMS NIH HHS [GM35433, GM40633, GM26357] Funding Source: Medline
We have obtained a 3D reconstruction of intact microtubules, using cryoelectron microscopy and image processing, at a resolution of about 8 Angstrom, sufficient to resolve much of the secondary structure. The level of detail in the map allows docking of the tubulin structure previously determined by electron crystallography, with very strong constraints, providing several important insights not previously available through docking tubulin into lower-resolution maps. This work provides an improved picture of the interactions between adjacent protofilaments, which are responsible for microtubule stability, and also suggests that some structural features are different in microtubules from those in the zinc sheets with which the tubulin structure was determined.
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