期刊
NATURE IMMUNOLOGY
卷 3, 期 10, 页码 918-925出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ni843
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资金
- NIAID NIH HHS [R01 AI009484, AI09484, R01 AI009484-32] Funding Source: Medline
- NIA NIH HHS [T32 AG000080, AG00080] Funding Source: Medline
Antigen-specific CD8(+) T cells are required for the clearance of most viral infections and several cancers. However, it is not clear in vivo whether CD8(+) T cells can engage multiple targets simultaneously, engagement results in the formation of an immunologic synapse or molecules involved in CD8 function are redistributed to the synapse. We used here high-resolution microscopy to visualize interactions between virus-specific effectors and target cells in vivo. Using either in situ tetramer staining or green fluorescent protein-labeled virus-specific T cells, we have shown that a single CD8(+) T cell can engage two or three targets, a synapse occurs at the site of engagement and molecules involved in attachment (lymphocyte function-associated antigen 1), signaling (Lck) and lytic activity (perforin) are differentially positioned on the T cell. In addition, we have established an in vivo approach for assessing the intricacies of antigen-specific T cell activation, migration, engagement, memory and other defining elements of adaptive immunity.
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