期刊
ANALYTICAL BIOCHEMISTRY
卷 309, 期 1, 页码 109-116出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0003-2697(02)00410-4
关键词
plasmon resonant particle (PRP); single nucleotide polymorphism (SNP); nanoparticle; fluorescence labels; ultrasensitive light-scattering labels
资金
- NCRR NIH HHS [2R42RR12415] Funding Source: Medline
- NIA NIH HHS [1R43AG186940] Funding Source: Medline
We demonstrate the use of silver plasmon resonant particles (PRPs), as reporter labels, in a micro array-based DNA hybridization assay in which we screen for a known polymorphic site in the breast cancer gene BRCA1. PRPs (40-100nm in diameter) image as diffraction-limited points of colored light in a standard microscope equipped with dark-field illumination, and can be individually identified and discriminated against background scatter. Rather than overall intensity, the number of PRPs counted in a CCD image by a software algorithm serves as the signal in these assays. In a typical PRP hybridization assay, we achieve a detection sensitivity that is similar to60x greater than that achieved by using fluorescent labels. We conclude that single particle counting is robust, generally applicable to a wide variety of assay platforms, and can be integrated into low-cost and quantitative detection systems for single nucleotide polymorphism analysis. (C) 2002 Elsevier Science (USA). All rights reserved.
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