期刊
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
卷 27, 期 4, 页码 436-445出版社
AMER THORACIC SOC
DOI: 10.1165/rcmb.2002-0012OC
关键词
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资金
- NHLBI NIH HHS [HL-60644, HL-67206, R01 HL066125, HL-55341, R01 HL066125-02, R01 HL066125-08] Funding Source: Medline
In ciliated airway epithelial cells, purinergic stimulation increases both intracellular calcium ([Ca2+](i)) and ciliary beat frequency (CBF). Because regulator of G-protein signaling protein 2 (RGS2) terminates Galphaq-mediated phospholipase C activation, we examined its role in regulating purinergic signaling in human and ovine airway epithelial cells. RT-PCR of both human and ovine epithelial cell RNA yielded fragments of expected size (similar to 491 bp) and sequence, confirming RGS2 message. Immunofluorescence demonstrated RGS2 protein expression in cultured airway epithelial cells of both species. Overexpression of an EGFP-RGS2 fusion protein (increasing RGS2 protein levels 1.8 times control, n = 28 cells) resulted in a reduced [Ca2+](i) and CBF response to 10 muM ATP (human: 58 +/- 9% and 49 +/- 8% lower, respectively; n = 8 measurements, 4 cells; ovine: 56 +/- 12% and 53 +/- 16% lower, respectively; n 5 measurements, 4 cells). Reducing RGS2 protein levels using antisense oligonucleotides increased the response of both [Ca2+](i) and CBF to ATP in human cells by 57 +/- 10% and 47 +/- 11%, respectively (n = 10 measurements, 6 cells), and in ovine cells by 88 +/- 13% and 48 9%, respectively (n = 10 measurements, 5 cells). These data provide functional evidence that RGS2 modulates purinergic signaling in human and ovine ciliated airway epithelial cells.
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