期刊
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT
卷 150, 期 10, 页码 564-568出版社
BLACKWELL VERLAG GMBH
DOI: 10.1046/j.1439-0434.2002.00795.x
关键词
Polymyxa graminis; subtractive hybridization; DNA cloning; polymerase chain reaction
A simple subtractive hybridization was applied for cloning of Polymyxa graminis deoxyribonucleic acid (DNA). Total DNA preparations from concentrated P. graminis resting spores and from roots of non-infested (healthy) barley were digested with different restriction enzymes and batch hybridized, followed by cloning in a plasmid vector. Sequencing and blast analysis of coincidentally selected clones enabled construction of polymerase chain reaction primers specific to P. graminis DNA. Four Polymyxa -specific primers showed different affinities to DNA of type I and type II P. graminis and to DNA of P. betae .
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