期刊
JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA
卷 7, 期 4, 页码 385-393出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1023/A:1024034031472
关键词
proteomics; 2D gel electrophoresis; differential protein expression; bioinformatics; clustering
The protein complement of breast cells consists of many thousands of proteins. Recent developments in 2D gel electrophoresis technology have made studies requiring the quantitative analysis of a differential proteome, such as comparison between normal and malignant cells or investigation of drug effects on cells, truly feasible. Computer software plays a central part in the comparisons between multiple gels required for such experiments. In addition, software tools allow patterns of coexpression of proteins to be studied, offering potential insights into protein regulation, interactions, and functions, especially when combined with complementary data on gene expression. In this paper, the technology and limitations of 2D gel-based proteomics are reviewed. Techniques for comparing sets of gels at a global level as well as identifying specific protein features that differentiate gels are discussed. Our own experience of studying the breast cell proteome is used to illustrate the difficulties and achievements of differential proteomics.
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