期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 12, 期 5, 页码 634-641出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/S0959-440X(02)00368-8
关键词
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Fluorescence correlation spectroscopy is a method in which fluctuations in the fluorescence arising from a very small sample volume are correlated to obtain information about the processes giving rise to the fluctuations. Recent progress has been made in methodologies such as two-photon excitation, photon counting histogram analysis, cross-correlation, image correlation and evanescent excitation. Fluorescence correlation spectroscopy techniques have been applied to several biological processes, including fluorescent protein photodynamics, binding equilibria and kinetics, protein oligomerization, nucleic acid interactions, and membrane and intracellular dynamics.
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