The therapeutic value of DNA-damaging antineoplastic agents is dependent upon their ability to induce tumor cell apoptosis while sparing most normal tissues. Here, we show that a component of the apoptotic response to these agents in several different types of tumor cells is the deamidation of two asparagines in the unstructured loop of Bcl-X-L, and we demonstrate that deamidation of these asparagines imports susceptibility to apoptosis by disrupting the ability of BcI-X-L to block the proapoptotic activity of BH3 domain-only proteins. Conversely, Bcl-X-L deamidation is actively suppressed in fibroblasts, and suppression of deamidation is an essential component of their resistance to DNA damage-induced apoptosis. Our results suggest that the regulation of Bcl-X-L deamidation has a critical role in the tumor-specific activity of DNA-damaging antineoplastic agents.
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