Phenobarbital blocks the lung edema induced by centrally injected tityustoxin in adult Wistar rats

The aim of this study was to evaluate the ability of phenobarbital to block the lung edema observed after intracerebroventricular (icv) injections of tityustoxin (TsTX); a toxic fraction of the Tityus serrulatus venom. We injected 1.74 mug icv (1.0 mul) of TsTX in Wistar rats pre-treated with 0.1 ml intramuscular injections of sterile saline or phenobarbital (60 or 170 mg/kg body weight). After the experiments the lungs were harvested and the pulmonary index (PI = lung/body weight x 100) calculated. The animals pre-treated with saline developed severe lung edema (PI = 1.8 +/- 0.2) after TsTX icv injection whereas those that received 170 mg/kg of phenobarbital presented no lung edema (PI = 0.71 +/- 0.02). Our results suggest that the lung edema induced by TsTX is of neurogenic nature and that 170 mg/kg of phenobarbital blocks TsTX induced lung edema. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.