期刊
JOURNAL OF CHROMATOGRAPHY A
卷 975, 期 2, 页码 275-284出版社
ELSEVIER
DOI: 10.1016/S0021-9673(02)01271-2
关键词
porosity; monolithic columns; inverse size-exclusion chromatography; silica C-18; polystyrene standards
Retention data of polystyrene samples of narrow molecular size distribution and known average molecular mass were measured on several monolithic columns (Chromolith Performance, Merck) and one conventional packed column (Luna C-18, Phenomenex) by size-exclusion chromatography. These data were used to determine the external, the internal, and the total porosities of these columns. These data provided also information on the pore-size distribution of the adsorbent medium. The external and the total porosities of these columns are much higher than those of conventional packed columns. The results illustrate the profound changes brought by monolithic columns to the balance of the hydrodynamic and the mass transfer kinetic properties of chromatographic columns. Classical methods of comparison between column performance must be re-evaluated. (C) 2002 Elsevier Science B.V. All rights reserved.
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