期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 32, 期 11, 页码 1525-1532出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0965-1748(02)00073-5
关键词
Dendroctonus jeffreyi; bark beetle; frontalin; pheromone biosynthesis; isoprenoid; 3-Hydroxy-3-methylglutaryl-CoA reductase; anterior midgut
资金
- NICHD NIH HHS [R01 HD038069] Funding Source: Medline
The male Jeffrey pine beetle, Dendroctonus jeffreyi Hopkins (Coleoptera: Scolytidae), produces the bicyclic ketal frontalin as part of a complex semiochemical blend. A key regulated enzyme in the mevalonate pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-R), showed high transcript levels in the anterior midgut of male Jeffrey pine beetles by in situ hybridization. HMG-R expression in this area of the alimentary canal was related to male emergence, where emerged males demonstrated significant up-regulation of HMG-R transcript and pre-emerged males showed only basal levels. Pre-emerged males were induced to express high levels of HMG-R transcript by treatment with juvenile hormone (JH) III. Additionally, isolated anterior midgut tissue from JH Ill-treated males converted radiolabeled acetate to frontalin, as assayed by radio-HPLC, providing strong evidence that this is the site of frontalin production in male beetles. (C) 2002 Elsevier Science Ltd. All rights reserved.
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