期刊
JOURNAL OF NEUROCHEMISTRY
卷 83, 期 4, 页码 934-945出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1471-4159.2002.01203.x
关键词
AMPA/KA receptors; cerebellar granule cell; excitotoxicity; GluR1; potassium
资金
- NINDS NIH HHS [NS38079] Funding Source: Medline
In addition to well-known N-methyl-D-aspartate (NMDA) receptor-mediated excitotoxicity, recent studies suggest that non-NMDA type ionotropic glutamate receptors are also important mediators of excitotoxic neuronal death, and that their functional expression can be regulated by the cellular environment. In this study, we used cerebellar granule cells (CGCs) in culture to investigate kainate (KA)-induced excitotoxicity. Although previous reports indicated that KA induces apoptosis of CGCs in culture, no KA-induced excitotoxic cell death was observed in CGCs treated with KA when cells were maintained in high potassium media (24 mM K+). In contrast, when mature CGCs were shifted into low potassium media (3 mM K+), KA produced significant excitotoxicity. In electrophysiological studies, the KA-induced inward current density was significantly elevated in CGCs shifted into low K+ media compared with those maintained in high K+ media. Non-desensitizing aspects of KA currents observed in this study suggest that these responses were mediated by AMPA rather than KA receptors. In immunofluorescence studies, the surface expression of GluR1 subunits increased when mature CGCs were shifted into a low K+ environment. This study suggests that KA-induced excitotoxicity in mature CGCs is dependent upon the extracellular potassium concentration, which modulates functional expression and excitability of AMPA/KA receptors.
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