期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 68, 期 11, 页码 5750-5755出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.68.11.5750-5755.2002
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资金
- NIGMS NIH HHS [GM56177] Funding Source: Medline
Production of coenzyme F-420 and its biosynthetic precursor FO was examined with a variety of aerobic actinomycetes to identify an improved source for these materials. Based on fermentation costs, safety, and ease of growth, Mycobacterium smegmatis was the best source for F-420-5,6. M. smegmatis produced 1 to 3 mumol of intracellular F-420 per liter of culture, which was more than the 0.85 to 1.0 mumol of F-420-2 per liter usually obtained with Methanobacterium thermoautotrophicum and similar to10-fold higher than what was previously reported for the best aerobic actinomycetes. An improved chromatography system using rapidly flowing quaternary aminoethyl ion-exchange material and Florisil was used to more quickly and easily purify F-420 than with previous methods.
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