期刊
CARBOHYDRATE POLYMERS
卷 50, 期 2, 页码 191-200出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0144-8617(02)00022-X
关键词
Eucalyptus wood; brewery's spent grain; preparative chromatography; xylo-oligosaccharides
Hydrolysates from two hydrothermally treated xylan-rich agrobased materials, Eucalyptus wood and brewery's spent grain were fractionated by anion-exchange chromatography and size-exclusion chromatography. Hereby, several pools were obtained and they were characterised by their sugar composition. Additionally, the oligosaccharides in the pools described were further identified by high-performance anion-exchange chromatography and mass spectrometry. The hydrothermally treated brewery's spent grain resulted in three pools of which two contained relatively high molecular weight xylan, singly and doubly branched with arabinose [XnAcm], separated from a pool of xylo-oligosaccharides less branched with arabinose. The fractionation of the hydrothermally treated Eucalyptus wood resulted in a neutral pool, mainly consisting of a series of (acetylated) xylo-oligosaccharides [XnAcm], and three acidic pools. Two of these acidic pools contained a series of (acetylated) xylo-oligosaccharides including one 4-0-methylglucuronic acid [X-n(GlcA(me))(1)Ac-m], while the third acidic pool contained (acetylated) xylo-oligosaccharides substituted with two 4-0-methylglucuronic acids [X-n(GlcA(me))(2)Ac-m]. Additionally, a series of xylo-oligosaccharides containing both 4-0-methylglucuronic acid and a hexose, most likely galactose, was detected in the acidic Eucalyptus pools [X-n(GlcA(me))(1 or 2)AcmH]. Information was obtained about the number of acetyl-groups linked to the (4-0-methylglucurono-) xylo-oligosaccharides. Finally, it is demonstrated with an example that the different substituents to the xylo-oligosaccharides present are of relevance for the fermentability of the xylo-oligosaccharides by human faecal samples. (C) 2002 Elsevier Science Ltd. All rights reserved.
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