期刊
PLASMID
卷 48, 期 3, 页码 174-178出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0147-619X(02)00104-X
关键词
P1 plasmid; partition; DNA segregation; cell division
The prophage of bacteriophage P I is a low copy number plasmid in Escherichia coli and is segregated to daughter cells by an active partition system. The dynamics of the partition process have now been successfully followed by time-lapse photomicroscopy. The process appears to be fundamentally different from that previously inferred from statistical analysis of fixed cells. A focus containing several plasmid copies is captured at the cell center. Immediately before cell division, the copies eject bi-directionally along the long axis of the cell. Cell division traps one or more plasmid copies in each daughter cell. These copies are free to move, associate, and disassociate. Later, they are captured to the new cell center to re-start the cycle. Studies with mutants suggest that the ability to segregate accurately at a very late stage in the cell cycle is dependent on a novel ability of the plasmid to control cell division. Should segregation be delayed, cell division is also delayed until segregation is successfully completed. (C) 2002 Elsevier Science (USA). All rights reserved.
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