期刊
EXPERIMENTAL PARASITOLOGY
卷 102, 期 3-4, 页码 157-163出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0014-4894(03)00051-1
关键词
Plasmodim falciparum; malaria; protozoa; HRP; histidine-rich protein; ELISA; enzyme-linked immunosorbent assay; drug sensitivity assay; drug resistance; IRBC; infected red blood cell; anti-malarial drug action
类别
Plasmodiumfalciparum histidine-rich protein II (HRP2) is one of the best documented malaria proteins. However, little is known about the development of HRP2 concentrations under the influence of anti-malarial drugs. HRP2 levels were determined in cell medium mixture. cellular compartment, and in culture supernatant using a double-site sandwich ELISA specific for HRP2. Characteristic increases in the overall HRP2 levels were found during the later ring and the trophozoite stages. Throughout the later schizont development, rupture, and reinvasion, however, the HRP2 levels remained comparatively stable. When the cultures were exposed to serial dilutions of anti-malarial drugs, a distinct inhibition of HRP2 production was seen with increasing concentrations of drugs, resulting in sigmoid dose-response curves, similar to those obtained from conventional drug sensitivity assays. HRP2 therefore allows for a very accurate estimation of parasite development and its inhibition and may therefore be ideally suited for use in drug sensitivity or bioassays. (C) 2003 Elsevier Science (USA). All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据