期刊
JOURNAL OF IMMUNOLOGY
卷 169, 期 9, 页码 4811-4821出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.169.9.4811
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资金
- NCI NIH HHS [R01 CA78263, R01 CA89511] Funding Source: Medline
Tumor-specific effector T cells (TE) are naturally sensitized within the L-selectin(low) (CD62(low)) fraction of tumor-draining lymph nodes (TDLN). Whether isolated from day 9 (D9) or day 12 (D12) TDLN, 5 million L-selectin(low) T-E could be culture activated and adoptively transferred to achieve complete rejection of established intradermal, pulmonary, and brain tumors. Surprisingly,, although 25 million unfractionated T cells from D9 TDLN were equally effective, even 100 million unfractionated T cells from D12 TDLN seldom prevented lethal intradermal tumor progression, despite a pronounced therapeutic excess of TE. This highly reproducible treatment failure was due to cotransfer of tumor-induced, L-selectin(high) suppressor T cells (T-S) which were also present in D12 TDLN. In contrast, D9 TDLN and normal spleens lacked L-selectin high T-S Only those L-selectin high D12 TDLN T cells that down-regulated L-selectin during culture activation were suppressive in vivo and in vitro, and, like L-selectin(low) T-E, trafficked promptly into tumors following i.v. administration. This is the first demonstration that adoptive immunotherapy can fail as a direct result of passenger T-S that share certain phenotypic and trafficking features of T-S even when otherwise curative doses of T-S have been administered. Furthermore, in contrast to recently described CD4(+)CD25(+) T-S and plasmacytoid dendritic cell-activated T-S, tumor-induced L-selectin high T-S prevent tumor rejection via blockade of sensitized, activated TE rather than via afferent blockade.
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