期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 22, 期 21, 页码 7385-7397出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.22.21.7385-7397.2002
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资金
- NCI NIH HHS [R01 CA064795, CA64795] Funding Source: Medline
- NIA NIH HHS [R01 AG004821, AG04821] Funding Source: Medline
- NIGMS NIH HHS [T32 GM007270, T32 GM07270] Funding Source: Medline
Human fibroblasts undergo cellular senescence after a finite number of divisions, in response to the erosion of telomeres. In addition to being terminally arrested in the cell cycle, senescent fibroblasts express genes that are normally induced upon wounding, including genes that remodel the extracellular matrix. We have identified the novel zinc finger protein APA-1, whose expression increased in senescent human fibroblasts independent of telomere shortening. Extended passage, telomerase-immortalized fibroblasts had increased levels of APA-1 as well as the cyclin-dependent kinase inhibitor p16. In fibroblasts, APA-1 was modified by the ubiquitin-like protein SUMO-1, which increased APA-1 half-life, possibly by blocking ubiquitin-mediated degradation. Overexpression of APA-1 did not cause cell cycle arrest; but, it induced transcription of the extracellular matrix-remodeling genes MMPI and PAI2, which are associated with fibroblast senescence. MMPI and PAI2 transcript levels also increased in telomerase-immortalized fibroblasts that had high levels of APA-1, demonstrating that the matrix-remodeling phenotype of senescent fibroblasts was not induced by telomere attrition alone. APA-1 was able to transactivate and bind to the MMPI promoter, suggesting that APA-1 is a transcription factor that regulates expression of matrix-remodeling genes during fibroblast senescence.
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