期刊
RESEARCH IN MICROBIOLOGY
卷 153, 期 9, 页码 585-592出版社
EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/S0923-2508(02)01372-4
关键词
Phoma herbarum; continental Antarctica; exopolysaccharide (beta-glucan) production; structural characterization; resistance to freezing
类别
The filamentous fungus Phoma herbarum CCFEE 5080 isolated from continental Antarctica soil was tested for exopolysaccharide (EPS) production. The fungus grew and produced EPS (up to 13.6 g/l) on a variety of carbon sources among which sorbitol was best, particularly at the concentration of 60 g/l. EPS production was maximum when the nitrogen source was NaNO3 (3 g/l) and the incubation temperature was 28 degreesC. The polysaccharide was purified by repeated precipitation in ethanol and gel filtration and characterized as a homopolymer of glucose having a molecular weight of 7.412 x 10(6); structural analysis indicated the presence of beta-1,3 and beta-1,6 linkages only. After repeated freezing and thawing of the fungal biomass in the presence of EPS, the mycelial growth was much higher than that observed after freezing in the absence of EPS and the difference increased with the number of freeze-thaw cycles. It is hypothesized that the adaptation of P. herbarum CCFEE 5080 to the Antarctic soil microclimatic conditions, characterized by low temperature, high thermal fluctuations and repeated freeze-thaw cycles, might be related to the EPS production ability. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
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