期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 66, 期 11, 页码 2491-2494出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.66.2491
关键词
neurite outgrowth; PC12 cell; nerve growth factor; protoberberine alkaloid; Coptidis Rhizoma
A methanol extract of Coptidis Rhizoma effectively enhanced the outgrowth of neurite in PC12 cells induced by nerve growth factor (NGF). Following solvent partition and preparative HPLC, berberine was isolated as the major active compound. Berberine enhanced the proportion of neurite-bearing cells in a dose-dependent manner without cytotoxicity. Its structural relatives, palmatine and coptisine, showed a slightly weaker NGF-enhancing effect than berberine. These three alkaloids inhibited acetylcholinesterase activity at a level comparable to that of physostigmine, but this inhibition was not responsible for the potentiation of NGF-induced neurite outgrowth. It is demonstrated for the first time that protoberberine alkaloids potentiated the NGF-induced differentiation of neural cells.
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