期刊
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
卷 130, 期 2, 页码 300-306出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-2249.2002.01972.x
关键词
antibodies; Fc gamma R; human; phagocytes; Plasmodium falciparum
类别
Although convincing evidence exists for the role of immunoglobulin G (IgG) antibodies in immunity to malaria, antibody titres do not usually predict protection. In this study we have assessed the interaction between Plasmodium falciparum- infected erythrocytes (PE), opsonized with immune serum containing different amounts of IgG antibody isotypes, with either THP-1 cells, ex-vivo human monocytes or IIAI.6 transfectant cells expressing Fcgamma RIIa-Arg/Arg131 or -His/His131 allotypes. Our results show that PMA-treated THP-1 cells were capable of phagocytosing serum-opsonized PE by Fcgamma RI (CD64) and Fcgamma RIIa (CD32), acting synergistically. The known Fcgamma RIIa polymorphism motivated us to examine its influence on IgG isotype-mediated phagocytosis of opsonized PE with human monocytes and the IIAI.6 transfectant cells expressing either allelic forms. Regardless of the cell type, PE phagocytosis with Fcgamma RIIa-His/His131 was highest following opsonization with a predominantly IgG3-containing immune serum pool. In contrast, PE phagocytosis with Fcgamma RIIa-Arg/Arg131 tended to be higher with an IgG1-containing pool. These results suggest a genetically determined influence of effector cell phenotype on IgG antibody-pathogen interaction in P. falciparum malaria.
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