Isolation of several anti-stress genes from a mangrove plant Avicennia marina

In order to isolate anti-stress genes from mangrove plants, a cDNA library of Avicennia marina was constructed and screened for anti-stress genes by a functional expression screening with Escherichia coli cells. Several stress-related gene homologues, such as chaperonin-60, clpP protease of the clp/Hsp100 family of chaperones, ubiquitin, eEF1A, drought-induced AtDi19 gene of Arabidopsis thaliana, and secretory peroxidase, were successfully isolated.