期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 51, 期 3, 页码 301-311出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(02)00106-9
关键词
checkerboard DNA hybridisation; dental caries; dental plaque; gram-positive species
资金
- NIDCR NIH HHS [R01-DE1272-O1A1] Funding Source: Medline
Checkerboard DNA-DNA hybridisation enabled the quantitative analysis of plaque samples against 40 microbial species simultaneously, using digoxygenin-labelled, whole-genome DNA probes. This technique was initially developed to study the predominantly Gram-negative sub-gingival plaque microbiota. The aim of this study was to apply it to a suite of predominantly Gram-positive microorganisms, such as those implicated in cariogenesis. To specifically target Grain-positive species (and Candida albicans) required optimisation and modification of DNA extraction, prehybridisation, hybridisation, and antibody detection conditions. The suitability of the revised technique for clinical and epidemiological studies was confirmed using interproximal plaque from small groups of 5- to 6-year-old children of high (decayed, missing, or filled teeth (dmft)greater than or equal to5, n=8) and zero (n=5) caries rates. (C) 2002 Elsevier Science B.V. All rights reserved.
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