期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 124, 期 44, 页码 13008-13017出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja025563k
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资金
- NCI NIH HHS [CA-65875] Funding Source: Medline
Evaluation of the sequence selectivity, noncovalent association, and orientation of the DNA cross-linking agent azinomycin B on its duplex DNA receptor is described. A strong correlation between sequence nucleophilicity and cross-linking yield was observed, and steric effects due to the thymine C5-methyl group were identified. Detailed studies on the role of the azinomycin naphthoate using viscometry, fluorescence contact energy transfer, and DNA unwinding assays point to a nonintercalative binding mode for this group. A kinetic assay for agent regioselectivity was used to determine the orientation of binding and covalent cross-link formation.
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