期刊
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
卷 49, 期 3, 页码 321-325出版社
WILEY
DOI: 10.1002/prot.10210
关键词
protein-protein interaction; Raf-1; Bad; phosphoserine
资金
- NIGMS NIH HHS [GM53165, GM60033] Funding Source: Medline
14-3-3 proteins are a family of conserved dimeric molecules that interact with a broad range of target proteins, most of which contain phosphoserine/threonine. The amphipathic groove of 14-3-3 is the main structural feature involved in mediating its associations. We have studied another domain of 14-3-3, the C-terminal loop, to determine what role it plays in ligand interaction. A truncated form of 14-3-3 lacking this C-terminal loop was generated and found to bind with higher affinity than the wild-type 14-3-3 protein to the ligands Raf-1 and Bad. Interestingly, the truncated 14-3-3 also showed increased association with the 14-3-3 binding-deficient Bad/S136A mutant. Taken together, these data support a role for the C-terminal loop as a general inhibitor of 14-3-3/ligand interactions. This may provide a mechanism by which inappropriate associations with 14-3-3 are prevented. (C) 2002 Wiley-Liss, Inc.
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