期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 40, 期 12, 页码 1061-1069出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/S0981-9428(02)01471-7
关键词
chitosan; Oryza sativa; oxidative stress; pathogenesis-related proteins and genes; phytoalexins; phytocystatins
In this study, we examined the response(s) of rice (Oryza sativa L. japonica-type cv. Nipponbare) seedling leaves treated with a fungal elicitor chitosan (CT). Small brownish necrotic spots (streaks) appeared in the interveinal regions on the leaf surface after treatment by 0.1% CT, over the cut control. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis further revealed strong induction of ascorbate peroxidase, and changes in phytocystatins (cysteine proteinase inhibitors). Using two-dimensional polyacrylamide gel electrophoresis, evidence is provided for the accumulation of two major classes of pathogenesis-related (PR) proteins, namely OsPR5 and OsPR10 in the leaves. In parallel, northern analyses revealed potent accumulation of the OsPR5 and OsPR10 mRNAs; a time- and dose-dependent expression, and a requirement for de novo protein synthesis was observed. Furthermore, CT-elicited changes were also accompanied by production of anti-fungal phytoalexins, the flavonoid sakuranetin and the diterpenoid lactone momilactone A, as determined by liquid chromatography-mass spectrometry/mass spectrometry analysis. Present results reveal for the first time the potency of CT in initiating multiple events linked with defense/stress response(s) in the leaves of whole rice plants. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
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