期刊
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
卷 283, 期 6, 页码 C1801-C1808出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00341.2002
关键词
injury; lung; transport
资金
- NHLBI NIH HHS [HL-57204] Funding Source: Medline
Mechanical ventilation with high tidal volumes has been shown to contribute to the formation or worsening of interstitial and alveolar edema. Previously we showed that application of large biaxial deformations in vitro perturbs the concentration and distribution of functional tight junction proteins in alveolar epithelial cells. Using a novel method, we determined that applied epithelial strain increases paracellular permeability in a dose- and rate-dependent manner. Primary rat alveolar epithelial cells were subjected to 12%, 25%, or 37% change in surface area (DeltaSA) cyclic equibiaxial stretch for 1 h. Cells were also stretched noncyclically at 25% DeltaSA for 1 h. During the experimental period, a fluorescently tagged ouabain derivative was added to the apical fluid. Evidence of binding indicated functional failure of the paracellular transport barrier. The percentage of field area stained was quantified from microscopic images. There was no significant evidence of basolateral fluorescent staining at 12% DeltaSA or at 25% DeltaSA applied cyclically or statically. However, cyclic stretch at 37% DeltaSA resulted in significantly more staining than in unstretched cells (P < 0.0001) or those stretched at either 12% (P < 0.0001) or 25% cyclic (P < 0.0005) or static (P < 0.05) DeltaSA. These results suggest that large cyclic tidal volumes may increase paracellular permeability, potentially resulting in alveolar flooding.
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