期刊
ELECTROPHORESIS
卷 23, 期 24, 页码 4053-4059出版社
WILEY-BLACKWELL
DOI: 10.1002/elps.200290020
关键词
counterion dye stain; ethyl violet; protein stain; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; zincon
A background-free, fast protein staining method in polyacrylamide gel electrophoresis using an acidic dye, zincon (ZC) and a basic dye, ethyl violet (EV) is described. It is based on the counterion dye staining technique that employs two oppositely charged dyes to form an ion-pair complex in staining solution. The selective binding of free dye molecules to proteins in acidic solution produces bluish violet-colored bands. It is a rapid and end-point staining procedure, involving only fixing and staining steps that are completed in 1-1.5 h. The detection limit of this method is 8-15 ng of protein that is comparable to the sensitivity of the colloidal Coomassie Brilliant Blue G (CBBG) stain. Due to its sensitivity and speed, this stain may be more practical than any other dye-based stains for routine laboratory purposes.
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