期刊
PLANTA
卷 216, 期 2, 页码 334-344出版社
SPRINGER
DOI: 10.1007/s00425-002-0895-1
关键词
Arabidopsis; development (akt2/3-1 mutant); phloem; potassium channel; sugar loading
资金
- Biotechnology and Biological Sciences Research Council [G17764] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [G17764] Funding Source: Medline
Members of the AKT2/3 family have been identified as photosynthate-induced phloem K+ channels. Here we describe the isolation and characterisation of an AKT2/3 loss-of-function mutant (akt2/3-1) from Arabidopsis thaliana (L.) Heynh. Micro autoradiography following (CO2)-C-14 incubation in the light revealed that a major fraction of (CO2)-C-14-derived photosynthates leaking out of sieve tubes appears not to be effectively reloaded (retrieval) into the phloem of the mutant. Using the aphid stylectomy technique we showed that the phloem sap of the mutant, lacking the phloem channels of the AKT2/3 type, contained only half the sucrose content of the wild type. Furthermore, the akt2/3 mutant exhibited a reduced K+ dependence of the phloem potential. Xenopus oocytes expressing the phloem sucrose/proton symporter depolarise upon sucrose application. When, however, the phloem channel was co-expressed - mimicking the situation in the sieve tube/companion cell complex - depolarisation was prevented. From our studies we thus conclude that AKT2/3 regulates the sucrose/H+ symporters via the phloem potential.
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