期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 374, 期 7-8, 页码 1199-1204出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-002-1638-7
关键词
Neem; Azadirachta indica; Azadirachtin; Fatty acids; HPLC; GLC
资金
- National Oilseeds and Vegetable Oils Development Board (NOVODB), Ministry of Agriculture, Government of India
A simple and economical method has been developed to estimate the azadirachtin content and fatty acid composition of neem kernels. Neem kernels are crushed and soaked overnight in ethanol. The extract obtained is analysed by HPLC after filtering through a 0.22 mu m membrane. The peaks are separated using acetonitrile-water (40:60) 1 mL min(-1) as the mobile phase on an RP-18 column and monitored at 214 nm. For the determination of fatty acid composition, the fatty acids are directly transmethylated in the kernel powder by heating with methanol-acetyl chloride-benzene (20:1:4, v/v) for 1 h in a water bath. The fatty acid methyl esters (FAMEs) obtained are extracted in hexane and analysed using GLC. The separation of the FAMEs is achieved using an RH-Wax column using temperature programming, 170-200 degrees C at 2 degrees min(-1). The peaks are detected using an FID. Both the methods do not require any clean up or defatting of seeds. This results in faster, easier and more economical sample preparation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据