期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 97, 期 8, 页码 1101-1105出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762002000800006
关键词
Trypanosoma cruzi; clone CL B5; biological characterization
Clone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered.
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