期刊
PEPTIDES
卷 23, 期 12, 页码 2203-2211出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0196-9781(02)00251-6
关键词
hypocretin; orexin; narcolepsy; enzyme immunoassay; histamine receptor; knockout
资金
- NINDS NIH HHS [NS23724] Funding Source: Medline
The hypocretins (1 and 2) have emerged as key regulators of sleep and wakefulness. We developed a high-throughput enzyme immunoassay (EIA) to measure total brain hypocretin levels from large numbers of mice. Hypocretin levels were not altered by circadian time or age. However, significant differences in one or both hypocretin peptides were observed between different mouse strains. We studied hypocretin levels in knockout and transgenic mouse models with obesity, circadian gene mutations or monoaminergic defects. Compared to controls, only histamine receptor knockouts had lower hypocretin levels. This was most pronounced in H1 receptor knockouts suggesting the existence of a positive feedback loop between hypocretin and histaminergic neurons. (C) 2002 Elsevier Science Inc. All rights reserved.
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