期刊
PLANT SCIENCE
卷 163, 期 6, 页码 1075-1082出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/S0168-9452(02)00273-X
关键词
Escherichia coli; chloroplast protein synthesis elongation factor (EF-Tu); heat stress; heat tolerance; Zea mays
A heat-tolerant maize line, ZPBL 1304, synthesizes increased amounts of chloroplast protein synthesis elongation factor (EF-Tu) under heat stress conditions. Previous studies have suggested that maize EF-Tu may be involved in the development of heat tolerance [Planta 212 (2001) 359; J. Plant Physiol. 153 (1998) 497]. In this study, we tested the hypothesis that overexpression. of maize EF-Tu enhances the viability of Escherichia coli under heat stress. The,approach was to expose E. coli transformed with a maize EF-Tu expression vector (pTrcHis2A-Zmeftul) to 55 degreesC and assess viability at 37 degreesC. Western blots showed E. call overproduced recombinant EF-Tu protein, and the protein seemed to be in a highly soluble form. E. coli overexpressing maize EF-Tu showed increased viability after exposure to heat stress, demonstrating that the maize EF-Tu is involved in the development of heat tolerance. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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