期刊
FERTILITY AND STERILITY
卷 78, 期 6, 页码 1225-1233出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0015-0282(02)04345-5
关键词
spermatogonial stem cells; xenogeneic stem cell transplantation; mouse Sertoli cell; immunohistochernistry; human fertility; cryopreservation; leuprolide
资金
- NICHD NIH HHS [HD-36504] Funding Source: Medline
- NIDDK NIH HHS [5-P30-DK-47747-01] Funding Source: Medline
Objective: To evaluate colonizing ability of human spermatogonial stem cells in mouse testes. Design: Transplantation of human testis cells into the seminiferous tubules of immunodeficient mice. Setting: University hospital and academic laboratory. Patient(s): Men with obstructive azoospermia or maturation arrest of spermatogenesis. Intervention(s): Analyzed up to 6 months after transplantation. Also analyzed: cryopreservation of donor cells, donor cell concentrations, and leuprolide treatment of recipients. Main Outcome Measure(s): Detection of human donor cells in recipient testes using whole-mount immunohistochemistry with antibodies that react with human germ cells. Result(s): Mouse testes were colonized by human testis cells obtained from each of 6 patients; overall, human spermatogonia were found in 16 of 22 (73%) recipient testes. Human spermatogonial stem cells survived in mouse testes for at least 6 months and proliferated during the first month after transplantation. No human-differentiating spermatogonia were identified, and meiotic differentiation did not occur in mouse testes. In this initial study, human stem cell colonization was not influenced by cryopreservation of donor cells, donor cell concentration, or leuprolide treatment of recipient mice. Conclusion(s): Xenogeneic transplantation of human germ cells using mice as recipients is feasible and could be used as a biological assay system to further characterize human spermatogonial stem cells. This study might provide a mechanism to evaluate the status of the stem cell population in selected infertile mate patients.
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