期刊
MOLECULAR ECOLOGY NOTES
卷 2, 期 4, 页码 456-458出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1471-8286.2002.00276.x
关键词
enrichment; microsatellites; paternity analysis; Poeciliidae; population
We isolated microsatellite loci for the Trinidadian guppy Poecilia reticulata from the Oropuche and Caroni drainages in Trinidad, using a modified enrichment protocol that provided 93% enriched libraries. The eight loci presented here are polymorphic, and have between seven and 18 alleles in 33-48 individuals initially screened. Observed heterozygosities in this sample range from 0.28 to 0.88. These loci significantly increase the number of polymorphic microsatellite markers available for paternity and population analysis in this species.
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