期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 32, 期 12, 页码 1643-1652出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0965-1748(02)00104-2
关键词
E75; nuclear receptor; follicular cells; oogenesis; Bombyx mori; 20E; ecdysone response
The steroid hormone 20-hydroxyecdysone (20E) plays a key role in the stimulation of ovarian follicle development in the silkmoth, Bombyx mori. To understand better the mechanism by which 20E regulates silkmoth oogenesis, Bombyx homologs of the ecdysone-inducible orphan nuclear receptor E75 (BmE75) were cloned and their expression was analyzed in developing ovaries and staged follicles during metamorphosis. Of the two BmE75 isoforms isolated, only the A-isoform (BmE75A) has been identified previously in lepidopteran insects. BmE75C, on the other hand, shows significant sequence homology in its N-terminus to the Drosophila E75C isoform. Northern blot analysis shows unique expression patterns for each isoform mRNA during ovarian development. While the A-isoform seems to be mainly implicated in the earlier stages of the ecdysone response during previtellogenesis and vitellogenesis, expression of the C-isoform becomes strongly induced in an ecdysteroid-independent fashion at the transition from vitellogenesis to choriogenesis. Our data indicate a complex regulation of the expression of the BmE75 gene during oogenesis and postulate a new role for the BmE75C receptor at the end of vitellogenesis and the beginning of choriogenesis. (C) 2002 Elsevier Science Ltd. All rights reserved.
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