期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 13, 期 6, 页码 557-564出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(02)00357-9
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Recent studies on microbial cytochrome P450 enzymes have covered several new areas. Advances have been made in structure-function analysis and new nonenzymatic/electrochemical systems for the replacement of NAD(P)H in biocatalysis have been developed. Furthermore, the properties of some enzymes have been re-engineered by site-directed mutagenesis or by methods of directed evolution and new P450s have been functionally expressed and characterized. It is thought that a combination of these approaches will facilitate the use of isolated P450 monooxygenases in biocatalysis.
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