3.8 Article

KV 2.1 K+ channels underlie major voltage-gated K plus outward current in H9c2 myoblasts

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JAPANESE JOURNAL OF PHYSIOLOGY
卷 52, 期 6, 页码 507-514

出版社

CENTER ACADEMIC PUBL JAPAN
DOI: 10.2170/jjphysiol.52.507

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outward K+ current; tetraethyl ammonium; quinidine; mRNA expression; H9c2 cell line

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The H9c2 clonal cell line derived from embryonic rat ventricle is an in vitro model system for cardiac and skeletal myocytes. We used the whole-cell patch clamp technique to characterize the electrophysiological and pharmacological properties of an outward K+ current (I-Kv) and determined its molecular correlate in H9c2 myoblasts. I-Kv, was activated by threshold depolarization to -30mV, and its current amplitude and rate of activation increased with further depolarizations. I-Kv inactivated slowly with a time constant of 1-2 s, and the V-0.5 for steady-state inactivation was -37.9 +/- 4.6 mV (n = 10). Tetraethyl-ammonium and quinidine suppressed I-Kv with IC50's of 3.7 mm and 11.6 muM, respectively. Using RT-PCR analysis we found that the K(v)2.1 gene is the most abundantly expressed among genes for K-v 1.2, 1.4, 1.5, 2.1, 4.2, and 4.3, and by Western blotting we confirmed the synthesis of the K(v)2.1 alpha-subunit protein. We conclude that IKv, the predominant voltage-gated outward current in H9c2 myoblasts, flows through the channels comprised of the K(v)2.1-subunit gene product.

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