期刊
NATURE REVIEWS MOLECULAR CELL BIOLOGY
卷 3, 期 12, 页码 964-970出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nrm975
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It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.
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