期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 13, 期 6, 页码 543-547出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(02)00350-6
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Lipases and esterases are frequently used in the synthesis of optically pure compounds; however, natural enzymes do not always show sufficiently high enantioselectivity. Variation of the structure of the substrates, modification of the reaction system or protein engineering (e.g. the expression of pure enzymes, rational design or directed evolution) are strategies that can be employed to improve the distinction between two enantiomers or enantiotopic groups.
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