期刊
FEBS LETTERS
卷 532, 期 1-2, 页码 75-79出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(02)03632-3
关键词
RNA silencing; suppressor; Tospovirus; Bunyaviridae; NSs; p19
RNA silencing or post-transcriptional gene silencing (PTGS) in plants is known as a defense system against virus infection. Several plant viruses have been shown to encode an RNA silencing suppressor. Using a green fluorescent protein-based transient suppression assay, we show that NSs protein of Tomato spotted wilt virus (TSWV) has RNA silencing suppressor activity. TSWV NSs protein suppressed sense transgene-induced PTGS but did not suppress inverted repeat transgene-induced PTGS. TSWV NSs protein is the first RNA silencing suppressor identified in negative-strand RNA viruses. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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