In vitro binding capacity of organic (wheat bran and rice bran) and inorganic (perlite) sources for Mn, Zn, Cu, and Fe

This study was carried out to determination of in vitro binding capacity of wheat bran, rice bran, and perlite for Mn, Zn, Cu, and Fe. For this reason, wheat bran and rice bran were chemically analysed for moisture, protein, ash, ether extract, total carbohydrate, neutral detergent fibre, acid detergent fibre, acid detergent lignin, crude fibre, cellulose, hemicelluloses, phytic acid, Non-Fiber Carbohydrate, total dietary fibre, soluble dietary fibre, insoluble dietary fibre, DF + lignin, and endogenous minerals. Mineral binding capacity of wheat bran, rice bran, and perlite for Mn, Zn, Fe, and Cu under sequential simulated physiological conditions of the stomach, small intestine, and colon was investigated and compared. Rice bran showed the highest mineral-binding capacity in small intestine for Mn, Zn, and Cu. Among the minerals, Mn exhibited the lowest percentage of binding under the simulated physiological conditions of the small intestine and the highest percentage of removing occurred in the stomach for all of the treatments. Cereal bran's had an affinity for Cu > Zn > Fe > Mn. The amounts of minerals bound varied among samples. Rice bran showed the highest and perlite had lowest mineral-binding capacity in small intestine for Mn, Zn, and Cu.