期刊
ORGANIC LETTERS
卷 4, 期 25, 页码 4459-4462出版社
AMER CHEMICAL SOC
DOI: 10.1021/ol0269897
关键词
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资金
- NIGMS NIH HHS [T32-GM08362] Funding Source: Medline
[GRAPHICS] Cycloartenol synthase cyclizes and rearranges oxidosqualene to the protosteryl cation and then specifically deprotonates from C-19. To identify mutants that deprotonate differently, randomly generated mutant cycloartenol synthases were selected in a yeast lanosterol synthase mutant. A novel His477Asn mutant was uncovered that produces 88% lanosterol and 12% parkeol. The His477Gln mutant produces 73% parkeol, 22% lanosterol, and 5% Delta(7)-lanosterol. These are the most accurate lanosterol synthase and parkeol synthase that have been generated by mutagenesis.
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