期刊
ANALYTICAL CHEMISTRY
卷 74, 期 24, 页码 6370-6377出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac0204000
关键词
-
资金
- NINDS NIH HHS [F32-NS11053, R01-NS42929] Funding Source: Medline
An indirect method for monitoring nitric oxide (NO) by determining nitrate and nitrite using microchip capillary electrophoresis (CE) with electrochemical (EC) detection has been developed. This method combines determination of nitrite by direct amperometric detection following a microchip-based CE separation and conversion of nitrate to nitrite by chemical reduction using Cu-coated Cd granules. The amount of nitrate is quantified by calculating the difference in the amount of nitrite in the sample before and after the reduction of nitrate. Optimization of the separation, injection, detection, and reduction reaction conditions, as well as studies involving integration of the reduction reaction onto the microchip, are described. It was found that nitrite can be separated and detected in similar to45 s by microchip CEEC. The reduction reaction was successfully integrated on-chip and carried out in similar to1 min following activation of the Cd granules. The usefulness of this device was demonstrated by monitoring the amount of nitrate and nitrite produced from 3-morpholinosydnonimine, a NO-releasing compound.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据