期刊
BRAIN RESEARCH
卷 958, 期 2, 页码 488-496出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0006-8993(02)03771-X
关键词
astrocyte; estrogen; estrogen receptor betas; glia, glial fibrillary acidic protein immunohistochemistry; interpeduncular nucleus
资金
- NCI NIH HHS [R25 CA 49425] Funding Source: Medline
- NINDS NIH HHS [NS42798, NS37795] Funding Source: Medline
We previously demonstrated that in rat, astrocytic glial fibrillary acidic protein- (GFAP) expression in the interpeduncular nucleus (IPN) was responsive to testosterone and in females the intensity of GFAP-immunoreactivity (IR) followed the periodic hormonal changes of the estrous cycle. The aim of this study was to test whether 17beta-estradiol (E-2), in the absence of other ovarian hormones, can influence GFAP-expression within individual subnuclei of the IPN and to determine the cellular distribution of estrogen receptor beta (ERbeta) in the IPN. Quantitative surface-density analysis,vas used to compare the intensity of GFAP-IR at different anterio-posterior (AP) levels of the IPN in ovariectomized female rats 24 h after treatment with E-2 or vehicle, Estrogen-treatment resulted in a significant increase in GFAP-IR in the rostrolateral subnucleus of the IPN at AP: -5.60, in the lateral-, dorsolateral-, dorsomedial- and central subnuclei at -6.04 and in the lateral subnucleus at -6.72. No significant differences were observed at -5.80 and -6.30. These results indicate that E,, in the absence of other ovarian hormones, modulates GFAP-expression within select IPN subnuclei and that these affects are dependent on position along the AP axis. To determine whether ERbeta was a possible mediator of the observed estrogenic effects, adjacent section pairs of the IPN were immunostained for ERbeta or GFAP. Using the 'mirror' method, ERbeta-IR was detected in the cytoplasm of GFAP-immunopositive astroglia and in the nuclei of GFAP-immunonegative neurons, These findings suggest that in the IPN, E-2 may directly modulate GFAP-expression through ERbeta-mediated mechanisms. (C) 2002 Elsevier Science B.V. All rights reserved.
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